Multiple context discrimination in adult rats: sex variability and dynamics of time-dependent generalization of an aversive memory

Table 1.

Summary of dimorphic and nondimorphic behaviors observed in the pilot study and main experiment

Dimorphic findings
Experiment Females Males
Pilot Discriminate P1/1′ < A P1/1′ =  A, due to higher fear in P1/1′
Main ABDC graded fear pattern different from males at 2 days ABCD graded fear at 2 days
Main Discriminate B from A at 2 days B equals A at 2 days
Main From discriminating A × B at 2 days to full generalization at 28/45 days B equals A at every test time interval
Main D = C < B < A (sign. differences) D < C < B = A (sign. differences)
Main Possible fear incubation in A from 2 to 28 days Unable to observe fear incubation due to ceiling effect
Main Partial generalization in D at 45 days Full generalization in D at 45 days
Main No flight behavior observed Flight behavior (jumps) observed
Main Do not discriminate floor texture at any time but seem to distinguish chambers by size at 2 and 28 days (PCA result) Do not discriminate floor texture at 2 days and chamber size at 2 and 28 days, but tend to reverse this with time (PCA result)
Pilot and Main Pilot study: freezing in A similar to males at 2 days Maximum freezing level in A at every test interval (with ceiling effect)
Main study; lower freezing in A at 2 days than at 28/45 days
Pilot and Main Better discriminators (lower fear to neutral contexts) Poorer discriminators
Nondimorphic findings
Experiment Both sexes
Main No sexual dimorphism is observed in three parameters—wall geometry, test session room, and cleaning agent scent (PCA result)
Main Ethanol is the dominant, most distinguishable scent at all times. Other cleaning agent scents tend to cluster according to sex at 45 days (PCA result)
Both sexes Recent memory Remote memory
Exhibit good memory acquisition (learning) and retention—with no deficit in consolidation (Fig. 2B,E) X X
Generalize partially or completely in one discrete step X
Converge to similar grouped levels of fear expression over time X
Do not display significant forgetting X
Do not exhibit darting behavior (but males jump/flight occasionally in some contexts) X X

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