Context discrimination of female and male rats 2, 28, or 45 days after training in a fear conditioning task. (A) Experimental protocol: all subjects were trained in Context A and subsequently tested either in A, B, C, or D 2, 28, or 45 days later. (B–D) Histograms showing mean ± SEM of the % freezing time in each context grouped by sex with individual dots overlaid representing individual animals. (E–G) Same data (and statistical analysis) grouped by context. (B–G) Data analyzed by independent two-way ANOVA followed by Tukey post hoc test. a (different from A/B), P < 0.015; b (differs from same sex groups tested in all other contexts), P < 0.002; c (different from A), P = 0.002; d (females differ from males in this specific context E), P < 0.0005; and e (different from A), P = 0.03. (H–J) Individual (and average) Z-scores for contexts B, C, and D (standard deviations compared to A).
