Detailed behavior analysis is integral to accurately capturing behavioral changes in omission. (A) Visualization of omission task. (B) Lever presses per minute (ppm) on the CS+ (teal) and CS− (orange) levers from the final Pavlovian conditioning session (12) through omission (13–17). Error ribbons display ± SEM. (C) Average food cup entries during CS+ presentations from the final Pavlovian conditioning session (12) through omission (13–17). Error ribbons display ± SEM. (D) Line plot of the PCA index change for each individual subject starting in the final Pavlovian conditioning session (session 12) through the final omission session (17). (E) Line plots of each individual animal's percentage of lever directed behaviors (behaviors from lever orient through lever bite in Fig. 1D vs. food cup directed and non-task directed behaviors) starting from the final Pavlovian conditioning session (session 12) through omission (sessions 13–17). (F) Line plots of each individual animal's sign-tracking intensity (behaviors in Fig. 1D scaled from −1 to 6, −1 representing food cup directed, and 6 representing lever bite) starting from the final Pavlovian conditioning session (session 12) through omission (sessions 13–17). (G) Scatterplot of the intensity of sign-tracking behavior and the amount of cancelled (deflected) trials. (H) Heatmap depicting the detailed behavioral fingerprints of animals in the final Pavlovian conditioning session (12). Darker blue regions indicate higher engagement with those behaviors, and lighter green to white represents the least engagement with those behaviors. Subjects are arranged in order of their calculated PCA index, from −1 to 1. PCA indexes of −0.5, 0, and 0.5 are marked by dotted black lines. (I) Heatmap depicting the detailed behavioral fingerprints of all animals in the final omission session (17). Darker blue regions indicate higher engagement with those behaviors, and lighter green to white represents the least engagement with those behaviors. Subjects are arranged in order of their calculated PCA index during this session (session 17), from −1 to 1. PCA indexes of −0.5, 0, and 0.5 are marked by dotted black lines. (J) Heatmap depicting the percent change in individual behaviors of all animals from session 12 to session 17. Orange indicates negative percent change, and blue represents positive percent change. Subjects are grouped by their initial, session 12 PCA-derived categorization (<−0.5: left; >−0.5 and <0.5: middle; >0.5: right), and ordered from −1 to 1 from left to right, as in H. For all plots, asterisks (*) represent statistical significance (P < 0.05).
