Social isolation decreases cell proliferation and adult hippocampal neurogenesis in females. (A) Representative images of BrdU immunostaining in the ventral DG of females in each group. Arrows indicate BrdU+ cells in the subgranular layer. (B) Social isolation did not affect the number of BrdU+ cells in the dorsal or ventral poles of males, but (C) decreased BrdU+ cells in the ventral pole of females. (D) Representative images of DCX immunostaining in the ventral DG of females in each group. (E) Social isolation did not affect the number of DCX+ cells in the dorsal or ventral poles of males, but (F) decreased DCX+ cells in the ventral pole of females. n = 7 control females, n = 7 stress females, n = 5–6 control males, n = 8 stress males. Data are represented as mean ± SEM. (*) P < 0.05. Scale bar, 200 μm.
