Prolonged PSE reduced the stimulus-evoked response. (A) Structure of PSE. (B) Mean (±SEM) anticipatory licking frequency for stimulus trials (green) and blank trials (red), and mean number of trials per day (gray shaded area). Blue shaded area indicates the training period. (C) Performance (Lstimulus − Lblank; mean ± SEM; see Materials and Methods) averaged across all mice. (D) Behavioral and imaging time line. Ten-day PSE started after 6-day ACC. (E) Significantly responsive activity evoked by vertical airpuff on ACC4–6. Averaged across six mice (mean ± SEM). (Middle and right) same as left, but for the averaged traces on PSE1 and PSE5, respectively. Gray shaded areas indicate the airpuff periods. (F) Peak airpuff-evoked response across the ACC and PSE period, averaged across mice and normalized to ACC4–6 (black, mean ± SEM; n = 6 mice). Gray lines indicate mean cell response for individual mice. One-way repeated measures ANOVA between ACC4–6 and PSE1–10, P = 1.5 × 10−5.
