SAT transiently enhanced neural activity in L2/3 Pyr. (A) Example traces of individual vertical-airpuff-evoked responses during acclimation day 4–6 (ACC4–6), SAT day 1 (SAT1), and SAT day 5 (SAT5). F (fluorescence) is reported in arbitrary units. The gray shaded area indicates the duration of airpuff. Only trials that were significantly responsive (peak response > 2 SD baseline) or ∼30% of all stimulus trials, were included in the analysis. (B, left) Significantly responsive activity evoked by vertical airpuff on ACC6. n = 9 mice. (Middle and right) Same as left, but for the averaged traces on SAT1 and SAT5, respectively. Mean ± SEM of shown in the figure. The gray shaded area indicates the duration of airpuff. (C) Mean response to airpuff across the ACC and training period, averaged across mice and normalized to ACC4–6 (black; n = 9 mice). Mean ± SEM of shown in the figure. Gray lines indicate the peak response of individual mice. One-tailed two-sample t-test: ACC4–6 versus SAT1, P = 0.063.
