Responses of L2/3 Pyr neurons were not specific to airpuff directions. (A) Schematic of airpuff setup under two-photon calcium imaging. (B, left) Averaged trace of the vertical-airpuff-evoked response during acclimation day 4–6 (ACC4–6). Only responsive trials were included. Trace was averaged across mice. Mean ± SEM of shown in the figure. Gray shaded area indicates the airpuff period. n = 5 mice. (Right) Same as left, but for horizontal-airpuff-evoked responses. (C) Correlation between peak response evoked by vertical and horizontal airpuffs within the same group of cells. Each dot represents one cell. The red dot indicates the mean response (vertical: mean ± SEM = 0.44 ± 0.10, horizontal: 0.43 ± 0.11; n = 139 cells). Pearson's correlation (Pearson's R = 0.81, P = 3.0 × 10−34).
