Synaptic GluA1 expression at the BLA correlates with fear generalization during testing. Differential centrifugation of BLA tissue taken from selected animals displaying varying degrees of testing pre-CS freezing yielded synaptic and extrasynaptic fractions containing PSD proteins. Quantification of these fractions via optical densitometry revealed dissimilar expression of synaptic (sGluA1 and sGluA2) (A,C) and extrasynaptic (eGluA1 and eGluA2) (B,D) AMPARs, with GluA1 showing the highest degree of linear correlation with pre-CS freezing duration (in seconds) during test. Squared Pearson coefficient (r2) and respective P-values are indicated as scatter plot insets. (E) Blots of all proteins analyzed on each fraction are shown, with pre-CS freezing levels displayed as phase percentage above the blots.
