High freezing and low rearing after first shock presentation predict fear generalization during test. Displayed are behavioral profiles of animals in clusters 1 and 2 for time (in seconds) spent freezing (A), rearing (B), and grooming (E), as well as average bout duration (in seconds per bout) of freezing (C) and absolute bout frequency of rearing (D) during training. We also show freezing duration (F), absolute bout frequency (G), and average bout duration (H) during test. We performed two-sample hypothesis testing using Student's t-test on parameters with normal distribution and Mann–Whitney U-test on nonnormal data (see Supplemental Table S15). Data points represent mean ± 1 SEM. Student's t-test: (**) P < 0.01, (***) P < 0.001; Mann–Whitney U-test: (#) P < 0.05, (###) P < 0.001.
