Sex-specific K48 polyubiquitin targets in the amygdala at 2 and 4 h after contextual fear conditioning. (A,B) Protein extracts from BLA samples were purified with K48 polyubiquitin TUBE and analyzed by liquid chromatography mass spectrometry and compared to naive age- and sex-matched controls. K48 polyubiquitin targets in males and females 2 h (A) and 4 h (B) after contextual fear conditioning (n = 5–6 per group). Data are displayed in heat maps with targeted proteins on the left and a colorimetric log2 fold change indicator on the right with sex being displayed on the bottom. Below the heat maps, data are summarized as Venn diagrams separated by either a significant positive (K48+) or negative (K48−) log2 value when compared to naive controls by sex. Data analysis conducted using FragPipe-Analyst with a DE log2 fold change >1 and P-value <0.05 being considered significant.
