Figure 2.
H2Aubi levels decrease at Pten following fear conditioning. (A) ChIP analysis revealed that fear conditioning resulted in decreased H2Aubi occupancy in the coding region of Pten (n = 8–9 per group, mixed sex, 8-week-old), but not in the promoter region (n = 9–10 per group, mixed sex) in the amygdala. (B) Fear-conditioned animals (n = 12, mixed sex, 8-week-old) had increased PTEN protein expression compared to the naive group (n = 11, mixed sex, 8-week-old). Western blot representative images of PTEN in the middle and β-actin (loading control) on the bottom. (*) P < 0.05 from Naive. (****) P < 0.0001 from Naive.
