Contextual fear transfer requires M1 mAChR activity in the CA1 region of the dHPC. (A) During the reactivation session, freezing levels did not differ between the three groups. Freezing behavior was measured as percent freezing (±SEM) across a 90 sec reactivation session. (B) There was no significant difference in freezing behavior during the post-reactivation session. Freezing behavior was measured as percent freezing (±SEM) across the 8 min post-reactivation session. (C) The Same-PIR group froze significantly less than the Same-VEH group during the test session, implying that PIR disrupted the updating of the fear memory to incorporate alternate contextual information. Freezing behavior was measured as percent freezing (±SEM) across the 8 min test session. (***) P < 0.001.
