Decoding Arc transcription: a live-cell study of stimulation patterns and transcriptional output

Calcium activity elicited by electrical burst stimulation with various burst frequencies. (A–E) Average ΔR/R profiles during burst stimulation at the frequency of 1 Hz (A), 5 Hz (B), 10 Hz (C), 15 Hz (D), and 20 Hz (E). (F) Average peak value of ΔR/R at each frequency (n = 7–19 cells, from three independent neuron cultures for each experiment, P = 0.0002 for one-way ANOVA test, (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, post hoc Tukey's t-tests). Error bars indicate SEM.