Knockdown of the H2Bub ligase Rnf20 does not alter performance during training. (A) Experimental time line. Male rats were injected with control (Scr-siRNA) or Rnf20-targeting (Rnf20-siRNA) Accell siRNAs into the CA1 region of the dorsal hippocampus. Five days later, animals were trained to a contextual fear conditioning procedure, euthanized 1 h later, and the dorsal CA1 region was collected for whole genome transcriptomic analysis. Separate animals were injected with Scr-siRNA and euthanized at an equivalent time without undergoing training and served as the naive control (n = 5 per group). (B) RT-PCR analysis confirmed a successful reduction in Rnf20 expression in the dorsal CA1 region 5 d after siRNA infusion. (C) Knockdown of Rnf20 did not impact performance during the contextual fear conditioning training session. (*) P < 0.05 from Scr-siRNA.
