mTORC1 activity in excitatory neurons versus inhibitory neurons during postnatal development to adulthood. (A) Representative immunoblots of hippocampus lysates taken from early postnatal (P10–15) and adult (P84–88) mice. (B–D) Quantification of (a). Number of mice, n = 8 per group, (**) P < 0.01, (****) P < 0.0001, calculated with an unpaired t-test. (E) Confocal microscopy images (scale bar, 50 μm, magnification 20×) of mouse hippocampus immunostained for GAD67 (red) and p-S6 (Ser240/244) (green) at P12 and P86. (F) Quantification (integrated density) of p-S6 (240/244) (green) from the CA1 pyramidal neuron layer of P12 and P86 mice, (**) P < 0.01, unpaired t-test. Data are presented as mean ± SEM. Number of mice, n = 4 per group. (G) Quantification (integrated density) of p-S6 (240/244) (green) from GAD67-positive neurons (red) of P12 and P86 mice; (ns) not significant. Data are presented as mean ± SEM. Number of mice, n = 4 per group.
