Remote fear memory recall activates a Fos ensemble uniformly distributed across CeA subdivisions. (A–C) Stereotaxic plate drawings of CeA rostro–caudal subregions (gray; left) along with representative examples of Fos-ir and corresponding summary distribution plots from context-TRAPed (middle) and fear-TRAPed (right) mice (n = 5/group) in the rostral (A), middle (B), and caudal (C) CeA subregions. Scale bars (context images: middle, left), 200 µm. (D) Average Fos-ir count density in each subregion. Two-way ANOVA with Sidak's posttest revealed no significant pairwise comparisons. Data are mean ± SEM. (E) Average Fos-ir count density in the rostral, middle, and caudal subregions of the CeAC and CeAML in context-TRAPed (light green) and fear-TRAPed (green) mice. Three-way ANOVA with Tukey's posttest revealed no significant pairwise comparisons. Data are mean ± SEM. (F) Correlation between the amount of time spent freezing during memory recall and Fos-ir counts in context (squares) and fear (circles) groups in the entire CeA (left), CeAC (middle), and CeAML (right). Least squares regression lines are shown along with corresponding Pearson's coefficients (r). Fisher's z-transformation was applied to compute r′ values comparing the strength of correlations between groups for the entire CeA and its CeAC and CeAML subdivisions (see Supplemental Table S2).
