Context fear conditioning triggers OGT-dependent changes in histone H3 K27 trimethylation. (A) Schematic of intra-CA1 siRNA infusion followed by training in contextual fear conditioning. (B) Quantification of histone western blots 1 h after training in context fear conditioning and OGT knockdown. (H2BubiK120: n = 7, 6, 6; H3K27me3: n = 7, 7, 6; H3K4me3: n = 5, 4, 4; H3K14ac: n = 7, 7, 6); all comparisons made via one-way ANOVA with Fisher's exact test, * denotes P < 0.05). (C) ChIP-qPCR at promoters of memory-related genes 1 h after training in contextual fear conditioning. (G9a: n = 4, 3, 5; Egr1: n = 4, 4, 5; GATA4: n = 4, 5, 5; Bdnf-VI: n = 3, 4, 4; Bdnf-IX: n = 3, 5, 5; Pkm-Zeta: n = 3, 5, 5; Comt: n = 4, 5, 5; Pten: n = 4, 5, 5; all comparisons made via one-way ANOVA with Fisher's exact test, * denotes P < 0.05).
