RPM and SunTag puncta colocalize with small and large ribosomal subunit markers. Shown is the colocalization of RPM (A,C) and V4-Arc 3′UTR (F,H) SunTag puncta with ribosomal protein S6 (B,G) and L19 (D,I) signal; puncta are indicated by white arrows. V4 (not shown) also colocalized with S6 and L19 as indicated by the quantifications in E and J; colocalization is represented as average Pearson's coefficients. A paired, two tailed t-test found no significant difference in colocalization of RPM signal with small and large ribosomal subunits; P = 0.3. Similarly, V4-Arc 3′UTR and V4 SunTag puncta both showed colocalization with the ribosomal subunit proteins; one-way ANOVA P = >0.5. N = 62 RPM and 48 SunTag neurite puncta from a combination of hippocampal and cortical neuron cultures. All errors are shown as standard deviation of the mean.
