SunTag and ribopuromycylation label the same initiation inhibitor resistant polysomes. Shown is the colocalization of V4-Arc 3′UTR (A) and V4 (D) SunTag puncta with RPM signal (B,E) (puncta are indicated by white arrows); quantification of V4-Arc 3′UTR-RPM and RPM-V4-Arc 3′UTR as well as V4-RPM and RPM-V4 colocalization, shown as average Pearson's coefficients, is included as C and F, respectively. Paired, two tailed t-tests found P values of 1 for V4-Arc 3′UTR-RPM and RPM-V4-Arc 3′UTR and 0.1 for V4-RPM and RPM-V4 comparisons. N = 72 RPM and 62 SunTag neurite puncta, taken from a combination of hippocampal and cortical neuron cultures. All errors are shown as standard deviation of the mean.
