Figure 5.
Emetine is not necessary for the ribopuromycylation assay. Emetine does not significantly change the number of RPM puncta (indicated by white arrows) in hippocampal (A,B) or cortical (D,E) neurons; quantified in C and F, respectively. A paired, two tailed t-test found P values of 0.3 and 0.2 for emetine vs. no emetine comparisons in hippocampal and cortical neurites, respectively. N = 66 emetine positive and 50 emetine negative hippocampal as well as 70 emetine positive and 52 emetine negative cortical neurites from multiple cultures. All errors are shown as standard deviation of the mean.
