Polysomes identified by live imaging of nascent peptides are stalled in hippocampal and cortical neurites

Comparison of distal and proximal dendrites. Border between proximal and distal parts of the neurite indicated by white line. Start (first cycle) and End (tenth cycle) throughout. SunTag puncta are indicated by white arrows. (A) A decrease in the density of proximal, but not distal, SunTag puncta is observed between the start and end of imaging in neuronal cultures treated with puromycin. (B) One-way ANOVA with Tukey's post-hoc multiple comparisons tests: F_(3,156) = 11.23, (****) P < 0.0001; NS, P > 0.2, n = 40. (C) No decrease in the density of proximal or distal SunTag puncta is observed between the start and end of imaging in neuronal cultures treated with H₂0. (D) One-way ANOVA with Tukey's post-hoc multiple comparisons tests, NS, P > 0.2, n = 41. (E) A decrease in the density of proximal, but not distal, SunTag puncta is observed between the start and end of imaging in neuronal cultures treated with HHT. (F) One-way ANOVA for HHT treatment with Tukey's post-hoc multiple comparisons tests: F_(3,216) = 11.83, (****) P < 0.0001; (**) P < 0.01; NS, P > 0.2, n = 55. (G) No decrease in the density of proximal or distal SunTag puncta is observed between the start and end of imaging in neuronal cultures treated with ethanol. (G) One-way ANOVA for ethanol treatment with Tukey's post-hoc multiple comparisons tests: NS, P > 0.2, n = 34.