Stagewise sequential maturation of the entorhinal-hippocampal network (Donato et al. 2017). (A) Schematic representation of information flow in the transverse entorhinal-hippocampal circuit. (B) Fraction of neurons with doublecortin (DCX) expression levels below the detection limit in each local network during successive days of maturation (DCX cumulative distributions, means ± SD). DCX is a microtubule-associated protein that is present in neuronal precursors and immature neurons, where it promotes dendritic growth and is down-regulated during the stabilization of synaptic connectivity at late developmental stages. (C) Hierarchical clustering of data in (B); x-axis length of the links of the dendrogram, arbitrary units. mEC layer (L) 2 striatal cells (MEC-L2 St) are the first population to exhibit dissimilarity from the rest of the network, followed by (i) MEC-L2 pyramidal (Pyr) and CA3, (ii) CA1, (iii) dentate gyrus, subiculum (SUB), MEC-L5, and LEC-L5, and (iv) LEC-L2.
