Figure 1.
The injection site of the siRNA. Taking the bregma as the coordinate origin, the injection site was: Anterior/Posterior: −2.5 mm; Medial/Lateral: ±2.4 mm; Dorsal/Ventral: −6.2 mm. (A) Verification of the injection sites by a fluorescent tracer. (Ai) shows the largest expand part of the LD at the coronal plane. (Aii) shows the most caudal portion of the LD at the coronal plane, (iii) and (iv) are schematic diagrams of (Ai) and (Aii), respectively. (B,C) PCR representative images and statistical analysis of the relative CREB mRNA level in each group. Lane 1: siCREB, lane 2: siScr, lane 3: LIPO. Error bars represent SD and significant differences are indicated by (*) P < 0.05, (**) P < 0.01. N = 9.
