Phospho-T840 immunodepletion assays using denatured homogenates prepared from hippocampal slices maintained in vitro. Hippocampal slices obtained from the same animal were either left untreated (UT) or exposed to 20 µM cantharidin (Can) for 1 h (n = 7). (A) Immunoblots showing phospho-T840 GluA1 levels in the input and unbound (UB) fractions from untreated control and cantharidin-treated slices. (B) Levels of T840-phosphorylated GluA1 in the input fractions from untreated control and cantharidin-treated slices. (C) Immunoblots show GluA1 in immunoprecipitated (IP) fraction (top) and unbound fractions (bottom) from untreated control and cantharidin-treated slices. (D) Levels of total GluA1 in the unbound fraction following immunoprecipitation of T840-phosphorylated GluA1 from homogenates prepared from untreated control and cantharidin treated slices. Lines in B and D connect results from the same experiment. Bars indicate median values.
