Immunoprecipitation of GluA1 from nondenatured and denatured hippocampal homogenates. (A) GluA2 subunits coimmunoprecipitate with GluA1 from nondenatured homogenates. Example immunoblots showing GluA1 and GluA2 levels in the input fraction, immunoprecipitated (IP) fraction (top), and unbound fraction (bottom) after immunoprecipitation with anti-GluA1 or control IgG antibodies. (B) Levels of GluA1 and GluA2 in the unbound fraction expressed as a percentage of levels in input fraction (n = 5, lines connect results from same immunoprecipitation). (C,D) GluA2 subunits do not coimmunoprecipitate with GluA1 from denatured homogenates. Same as in panels A and B except that GluA1 immunoprecipitations were performed using denatured homogenates (n = 5). Bars indicate median values.
