More remote fear memories were also susceptible to reconsolidation blockade induced by prelimbic (PL) cortex inactivation. One (A) or 3 (B) weeks after the contextual conditioning session in Context A (described in Figure 2), the animals were briefly reexposed to this chamber to reactivate the established fear memory, and immediately after they received vehicle (VEH) or muscimol (MUS, 4.0 nmol/side). In either case, MUS-treated animals froze less than controls when reexposed to the paired context 1 d later (Test A). No differences between groups were observed in an unpaired context (Test B). Gray arrowheads indicate the moment of drug treatment. Bars represent the percentage of total time spent freezing. Values are expressed as mean ± SEM. (*) Significant difference (P < 0.05) compared with the respective controls (repeated-measures ANOVA followed by Newman–Keuls test).
