ADX47273 enhanced reversal learning in the MWM. (A) Schematic of experimental design for MWM. D1–D3 animals were trained with a visible platform. D4–D10 mice were subjected to a hidden platform test. For D11 and D12, the platform was moved to the opposite quadrant, and mice were trained in reversal learning for two consecutive days with a total of seven trials. Finally, the platform was again moved to the opposite quadrant and mice were allowed seven trials for 2 d to relearn the new location (D13 and D14). Twenty to 30 min prior to each reversal trial, animals were injected with ADX47272 or vehicle. Three probe tests were conducted on D11, D13, and D15 at the beginning of each day (data not shown). (B, C) Escape latency of the mice during each day of the visible platform (B) and hidden platform test (C). (D) Escape latency during the reversal test. Drug group, n = 13; vehicle group, n = 12. (#) Two-way RM ANOVA, P < 0.05. (E) Swim speeds during the reversal test.
