SKF82958-induced expression of c-Fos immunoreactivity in the prelimbic cortex of animals that have been characterized for their GCAs. (Left) Schematic illustration of the regions of interest (marked by an arrow) for the prelimbic cortex (center arrow), agranular insular cortex (in a cross-section taken 5.9 mm rostral to the interaural line, left arrow), and the dorsomedial striatum (located 4.48 mm rostral to the interaural line, right arrow). Coordinates conform to Franklin and Paxinos (1997). (Right) Marked differences in the expression of c-Fos immunoreactivity were detected in the prelimbic cortex 60 min after SKF82958 administration between animals of high GCAs (A) when compared to animals of low GCAs (C). No measurable difference was observed between animals of high GCAs (B) and low GCAs (D) when administered saline. No c-Fos immunoreactivity was observed in a positive control (E).
