Figure 2.
LTP induction increases SGK phosphorylation, and SGKS422A transfection impairs the expression of LTP. (A) High-frequency stimulation of the Schaffer collateral/commissural pathway induces LTP at different time points. (T) Train of stimulation. (B) Western blot showed that SGK phosphorylation at Ser422 was significantly increased 3 h and 5 h after LTP induction (both P < 0.05, one-way ANOVA followed by Dunnett’s t-test). N = 4–6 each group. (C) SGKS422A transfection 48 h before tetanic stimulation markedly impaired the expression, but not the induction, of LTP in hippocampal neurons (P < 0.01, Student’s t-test). N = 5 each group. Data are mean ±SEM.
